I dont know if there is an option for that but the way I do it is when i setup the vendor, in the CONTACT line I enter the Account number and make sure that the "print Contact on cheque" option is selected that way when i print it will look like this:

Monkeys Cleaning Services
Acc# 1323546613
123 Planet Earth
Universe, Canada

If you need the contact NAME also, just use one of the first Address Line for either the name or the Acc# as you can start entering the Address in the Second Address line (obviously it might not work for an address that needs several lines but i have yet gotten that problem)

Sorry I should have been more specific, not the account numbers for the vendors themselves, but the account numbers from the chart of accounts, something similar to a GL entry I guess is what I'm asking if they could go on there.

The only way to do this in the program is to use Payments and enter it manually on the Comment line, then customise the cheque itself to remove the comment from the cheque face.

Can I ask why you would want it? I've seen a similar option in QB but never used it on files I set up and never needed the info on the stubs when I have seen it.